Etiltest - alcool test

Product Description

Rapid Test Etil Test is an enzymatic test for the rapid and safe detection of the presence of alcohol (alc) (ethyl alcohol/ethanol) in saliva and other liquid samples.

Features

By analyzing the saliva sample, it is possible to determine the concentration of alcohol in the blood. With this enzymatic analysis, a qualitative result is obtained, that is, the test shows the presence or absence of alcohol in the samples. The analysis detects an ethanol concentration of 0.1 or equal to 10 mg/dL, which is visualized by a light green-gray coloration of the reactive pad or test pad. The green becomes more intense as the concentration of alcohol in the sample increases. Therefore, in the presence of alcohol in the sample, Etil Test determines a color change that goes from light green-gray in the presence of an alcohol concentration of 0.1 mg/dL, changing to a slightly darker green in the presence of an alcohol concentration of 10 mg/dL. The color varies from intense with an average concentration, to a dark green-gray color if the alcohol concentration approaches 1.50.
Use this visual test only as a qualitative test, as the discrimination ability of the human eye can cause errors in terms of quantitative results.
It measures 5 levels of alcohol in the body: 0.00 g/l - 0.20 g/l - 0.50 g/l - 0.80 g/l - 1.50 g/l.
The Ethyl Test consists of a plastic stick with a reactive pad at the end. The pad, upon contact with alcohol solutions, quickly turns green to blue, depending on the amount of alcohol contained in the liquid. The reactive pad reacts based on a solid-phase chemical synthesis.

Interpretation of Results

Read the color of the reactive pad after 2 minutes. Do not compare the test result with other color scales. Use only the color scale shown on the package. The reaction occurs more slowly in saliva than in other aqueous solutions.

Limitations

If you do not wait at least 15 minutes after putting food, drinks, or other things in your mouth before performing the test, the results may be incorrect due to possible contamination of the saliva with interfering substances.
The Etil Test was designed and calibrated to be interpreted 2 minutes after soaking the reactive pad. If you wait more than 2 minutes, the results shown may be incorrect or false positive results may occur.
The Etil Test can be used to detect the presence of alcohol in fluids other than saliva. However, when used in this way, the color scale on the packaging cannot be used as a guide.
If alcohol is present in the fluid, the color of the swab will range from light green-gray to black or cocoa brown as the alcohol concentration increases. If the swab is immersed in pure alcohol, the swab will turn slightly or not at all colored due to the absence of water, which is necessary for the coloring reaction to occur.
When analyzing beverages, the result can only be considered positive if the swab turns very dark brown.
The Ethyl Alcohol Test is a very sensitive test for alcohol. Alcohol vapors, often present in the air, are sometimes detected by the Ethyl Alcohol Test.
Alcohol vapors are often present in many public places and inside homes. Alcohol is found in many household cleaning products such as disinfectants, deodorizers, and glass cleaners. If the presence of alcohol vapors is suspected, the test should be performed in an area where these vapors are not present (outdoors, for example).
Note: The person reading the test results cannot be color blind.

Controls

The integrity of the Alcohol Test can be qualitatively verified using a solution prepared by adding 4 drops of 40% alcohol by volume distilled liquor to a glass of water.
With this solution, the color should correspond to the 0.04% or higher window (darker).
The color reaction with alcohol in saliva occurs more slowly and with a less intense color than with alcohol in aqueous solutions.

Specificity

The Alcohol Test will react in the presence of methyl, ethyl, and allyl alcohol. Etil Test will not produce any reaction with alcohols containing 5 or more carbon atoms, nor with alcohols containing 5 or more carbon atoms. with glycine, glycerol, or serine.
This property derives from the specificity of the alcohol oxidase enzyme extracted from yeast.

Interactions

The following substances may interfere with the Ethyl Test stick if liquid samples other than saliva are used:

Color-enhancing agents:

• Peroxide.
• Strong oxidants.
• (common in detergents, pickling agents, and bleaching agents).

Color-inhibiting agents:

Reducing agents:

• Ascorbic acid.
• Tannic acid.
• Pyrogallol.
• Mercaptans and tosylates.
• Oxalic acid.
• Uric acid.
• Bilirubin.
• L-dopamine.
• L-methyldopamine.
• Metampirone.

These substances are usually not present in saliva in quantities sufficient to interfere with the test. However, it is necessary that they are not introduced into the mouth in the 15 minutes preceding the test.

Composition

Complete composition of the kit:

• Test strip: contains a filter paper pad with alcohol oxidase, peroxidase, 3,3,5,5-tetramethylbenzedine (TMB) and dry TRIS-HCl buffer.
• The test strip consists of: PE catalyst, filter paper reagent pad.
• Other components: aluminum bag, hygroscopic silica gel.
• Harmful components: none.

Directions for use

1. Do not introduce anything into the mouth in the fifteen (15) minutes preceding the start of the test. This warning includes non-alcoholic beverages, tobacco products, coffee, breath mints, food, etc.
2. Bring the sealed pouch to room temperature (15-30°C) to avoid condensation of moisture on the test pad.
3. If testing liquids other than saliva, ensure that the liquids are at room temperature before testing.
4. Open the pouch and remove the test stick. Observe the test pad at the end of the test stick. The pad should be a light cream color. A test stick with a dark brown or stained test pad should be discarded.
5. Soak the test pad with liquid or saliva collected from the mouth or a spittoon. Start the timer immediately.
6. After two (2) minutes, observe the color change (if any) on the test pad. If the swab turns green or dark green-gray, the test has detected the presence of alcohol and the result is positive. Results obtained after more than 3 minutes may be incorrect.
7. Estimate the approximate blood alcohol concentration by comparing the color of the reactive swab to the color scale on the test package. We recommend using the visual test only as a qualitative test.

Warnings

• Single use only.
• This product is not intended for medical or diagnostic purposes.
• Do not use beyond the expiration date.
• Allow the reactive pad to absorb enough liquid to completely soak it.
• Do not eat, drink or smoke while handling the samples or the kit.
• Do not use the test if the pouch is damaged.
• Bring the reagents to room temperature (15-30°C) before use.
• Do not touch the reactive pad on the stick to avoid contaminating it.
• Avoid cross-contamination of samples by using a new collection container for each sample.
• Not for medical or diagnostic use.
• Handle all samples as if they contained infectious agents. During the test, observe precautions against microbiological hazards and follow standard procedures for proper disposal of specimens.
• Leave the test stick in the sealed pouch until use.
• Observe the test result after 2-3 minutes.
• Humidity and high temperatures may affect the test results.
• Do not perform the test in an atmosphere containing alcohol.
• Pure alcohol may give false results.
• Be aware of possible cross-reactivity.
• We recommend using the visual test only as a qualitative test.
• The person reading the results cannot be color blind.

Storage

The test kit should be stored in the refrigerator or at room temperature (2-30C°) in the sealed pouch, depending on the shelf life of the test kit. Product.
Bring the test stick to room temperature to avoid condensation on the test pad.

Format

1 test